Lamb J R, Eckels D D, Phelan M, Lake P, Woody J N
J Immunol. 1982 Mar;128(3):1428-32.
Human T lymphocyte clones (TLC) specific for type A (A/Texas/1/77) influenza virus and maintained in continuous culture with T cell growth factor, were analyzed to define the cellular specificity pattern of virus recognition. A panel of TLC were stimulated with strains of serologically characterized type A influenza subtypes. Five TLC recognized all the viral subtypes; the remaining clones recognized only subtypes that shared serologically defined determinants with the immunizing subtype. In addition, the 11 TLC were analyzed for their fine antigenic specificity by using the purified viral components hemagglutinin (HA), neuraminidase (NA), matrix protein (MP), and nucleoprotein (NP). Five TLC proliferated in response to NA, four to MP, one to HA, and one to NP. None of the clones responded to the unrelated B strain influenza virus, B/Singapore. Furthermore, the fine specificity of an MP-reactive TLC was confirmed by subcloning.
对那些针对甲型（A/德州/1/77）流感病毒且在T细胞生长因子作用下持续培养的人T淋巴细胞克隆（TLC）进行分析，以确定病毒识别的细胞特异性模式。用血清学特征明确的甲型流感病毒亚型毒株刺激一组TLC。五个TLC识别所有病毒亚型；其余克隆仅识别与免疫亚型具有血清学定义决定簇相同的亚型。此外，通过使用纯化的病毒成分血凝素（HA）、神经氨酸酶（NA）、基质蛋白（MP）和核蛋白（NP），对这11个TLC进行了精细抗原特异性分析。五个TLC对NA有增殖反应，四个对MP有反应，一个对HA有反应，一个对NP有反应。没有一个克隆对无关的乙型流感病毒B/新加坡有反应。此外，通过亚克隆证实了一个对MP有反应的TLC的精细特异性。
