Combie J, Blake J W, Nugent T E, Tobin T
Res Commun Chem Pathol Pharmacol. 1982 Jan;35(1):27-41.
We have investigated the action of five sources of beta-glucuronidase enzymes on the hydrolysis of glucuronides of apomorphine, butorphanol, hydromorphone, nalbuphine, oxymorphone and pentazocine in equine urine. For all glucuronides tested, Patella vulgata beta-glucuronidase yielded the largest thin layer chromatographic (TLC) spots. For oxymorphone, P. vulgata was the only treatment to yield detectable TLC spots under test parameters. For these six drugs, TLC spot size and chromatographic quality were compared between control horses and horses pretreated with furosemide four hours earlier. Furosemide pretreatment produced a statistically significant increase in spot size and was found to enhance chromatogram quality. These findings support previous suggestions that P. vulgata is a superior drug-glucuronide hydrolyzing enzyme. They also support earlier reports that administration of furosemide at four hours pre-race is unlikely to result in significant interference with routine drug testing procedures.
我们研究了五种β-葡萄糖醛酸酶来源对马尿中阿扑吗啡、布托啡诺、氢吗啡酮、纳布啡、羟吗啡酮和喷他佐辛葡萄糖醛酸苷水解的作用。对于所有测试的葡萄糖醛酸苷，普通滨螺β-葡萄糖醛酸酶产生的薄层色谱（TLC）斑点最大。对于羟吗啡酮，在测试参数下，普通滨螺是唯一产生可检测TLC斑点的处理方法。对于这六种药物，比较了对照马和提前四小时用速尿预处理的马之间的TLC斑点大小和色谱质量。速尿预处理使斑点大小在统计学上显著增加，并发现可提高色谱质量。这些发现支持了之前关于普通滨螺是一种优质药物-葡萄糖醛酸苷水解酶的建议。它们还支持了早期的报告，即在赛前四小时给予速尿不太可能对常规药物检测程序造成重大干扰。
