Oud P S, Zahniser D J, Raaijmakers M C, Vooijs P G, Van de Walle R T
Anal Quant Cytol. 1981 Dec;3(4):289-94.
A staining method developed for use with BioPEPR (Biological Precision Encoding and Pattern Recognition), an automated image-analysis system for cervical smears, is described. The stain is a combination of the Feulgen procedure, with thionine-SO2 as the Schiff reagent, and Congo red, which is used as a counterstain. The stain resulted in smears suitable both for microscopic diagnosis and for BioPEPR measurements made on photonegatives at a single wavelength 545 nm. A high level of reproducibility and accuracy of nuclear and cytoplasmic area measurements was obtained. Nuclear integrated optical density could be well measured and was shown to be useful in discriminating between normal and abnormal cells. Using a combination of morphologic features, a high level of cell classification accuracy was reached. The possibility of using the stain for more detailed studies is discussed.
本文描述了一种为配合BioPEPR（生物精确编码与模式识别）开发的染色方法，BioPEPR是一种用于宫颈涂片的自动图像分析系统。该染色剂是福尔根程序的组合，使用硫堇-SO2作为席夫试剂，以及刚果红作为复染剂。该染色剂使涂片既适合显微镜诊断，也适合在545nm单一波长下对负片进行BioPEPR测量。获得了高水平的细胞核和细胞质面积测量的重现性和准确性。细胞核积分光密度能够得到很好的测量，并显示在区分正常细胞和异常细胞方面很有用。结合形态学特征，达到了高水平的细胞分类准确性。还讨论了将该染色剂用于更详细研究的可能性。
