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关于小鼠肿瘤病毒群特异性抗原gs-1的异质性(作者译)

[About the heterogeneity of the murine oncornavirus group-specific antigen gs-1 (author's transl)].

作者信息

Denner J, Dorfman N A

出版信息

Arch Geschwulstforsch. 1976;46(3):176-82.

PMID:61741
Abstract

In isoelectric focusing of twofold gradienpturified and Tween 80-ether disrupted RLV the groupspecific antigen (gs-1) was found in 3 pH-zones (4,45-5,5; 5,65-6,0; 6,45-6,6). For the gs-1 from plasma und spleen cells of leukemic mice was shown heterogeneity by several methods: 1. Gs-1 from plasma showed in isoelectric focusing a scattering along the whole elution profile. 2. Agarimmunoelectrophoresis resulted in a longer precipitationline for gs-1 from plasma in comparison with gs-1 from spleen cells. 3. Polyacrylamidgelelectrophoresis with following immunodiffusion made evident the particularly discrete character of this heterogeneity: gs-1 from both materials were found in two main zones.

摘要

在对双重梯度纯化且经吐温80-乙醚裂解的逆转录病毒样颗粒(RLV)进行等电聚焦时,发现群特异性抗原(gs-1)存在于3个pH区(4.45 - 5.5;5.65 - 6.0;6.45 - 6.6)。通过多种方法显示,白血病小鼠血浆和脾细胞中的gs-1具有异质性:1. 血浆中的gs-1在等电聚焦中沿整个洗脱图谱呈散射状。2. 琼脂免疫电泳显示,与脾细胞中的gs-1相比,血浆中的gs-1沉淀线更长。3. 聚丙烯酰胺凝胶电泳及随后的免疫扩散表明了这种异质性的特别离散特征:两种材料中的gs-1都存在于两个主要区带中。

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