Garcia-Segura L M, Baetens D, Orci L
Brain Res. 1982 Feb 25;234(2):494-9. doi: 10.1016/0006-8993(82)90893-9.
Vibratome slices of cerebellar cortex fixed in a glutaraldehyde solution containing filipin, a sterol-specific probe, were freeze-fractured to study the distribution of filipin-sterol complexes in neuronal plasma membranes. These complexes appear as 25-30 nm protuberances or pits in the fracture face of plasma membranes, and their density was low in dendrites of Purkinje and granule cells. In contrast, the plasma membranes of neuronal perikarya showed an abundant filipin labeling, 4-6 times greater than in dendrites. Parallel fibers, the axons of granule cells also had significantly more filipin-sterol complexes than granule cell dendrites, but fewer than granule cell perikarya. The results reveal difference in the organization of specific regions of neuronal plasma membranes, which are also characterized by a different pattern of synaptic contacts.
将固定在含有菲律宾菌素（一种固醇特异性探针）的戊二醛溶液中的小脑皮质振动切片进行冷冻断裂，以研究菲律宾菌素 - 固醇复合物在神经元质膜中的分布。这些复合物在质膜的断裂面上表现为25 - 30纳米的突起或凹陷，其密度在浦肯野细胞和颗粒细胞的树突中较低。相比之下，神经元胞体的质膜显示出丰富的菲律宾菌素标记，比树突中的标记多4 - 6倍。平行纤维，即颗粒细胞的轴突，其菲律宾菌素 - 固醇复合物也明显多于颗粒细胞树突，但少于颗粒细胞胞体。结果揭示了神经元质膜特定区域组织的差异，这些区域也具有不同的突触接触模式。
