Ware J L, Paulson D F, Parks S F, Webb K S
Cancer Res. 1982 Apr;42(4):1215-22.
The monoclonal antibody alpha Pro3 recognizes an antigen concentrated in human primary prostatic carcinoma tissue removed surgically. Although the antigen was detectable in extracts of human normal and malignant nonprostatic tissue, as well as in benign prostate tissue, quantitative absorption analysis revealed a substantially greater quantity of the antigen in malignant prostate tissue. The antigen recognized by alpha Pro3 in primary prostatic carcinoma has an apparent nonreduced molecular weight of 175,000 and an apparent subunit molecular weight of 54,000. This antigen, p54, appears to be present on the surface of cultured prostatic tumor cells of the PC-3 cell line, but its location in vivo has not been defined. Successful competition between alpha Pro3 and prostatic carcinoma patient serum immunoglobulin for a Mr 54,000 antigen (reduced molecular weight) present in prostatic carcinoma tissue extract suggests that p54 may play a significant role in the immunobiology of prostatic carcinoma. alpha Pro3 has potential as a sensitive probe for an antigen relevant to human tumor biology.
单克隆抗体α Pro3识别一种集中于手术切除的人原发性前列腺癌组织中的抗原。虽然在人正常和恶性非前列腺组织提取物以及良性前列腺组织中可检测到该抗原，但定量吸收分析显示恶性前列腺组织中该抗原的量要多得多。α Pro3在原发性前列腺癌中识别的抗原，其非还原表观分子量为175,000，亚基表观分子量为54,000。这种抗原p54似乎存在于PC - 3细胞系培养的前列腺肿瘤细胞表面，但其在体内的位置尚未明确。α Pro3与前列腺癌患者血清免疫球蛋白在前列腺癌组织提取物中存在的分子量为54,000的抗原（还原分子量）上成功竞争，表明p54可能在前列腺癌的免疫生物学中起重要作用。α Pro3有潜力作为一种与人类肿瘤生物学相关抗原的敏感探针。
