Baron M H, Baltimore D
Cell. 1982 Feb;28(2):395-404. doi: 10.1016/0092-8674(82)90357-9.
The genome-linked protein (VPg) of poliovirus has been chemically synthesized, coupled to bovine serum albumin carrier and injected into rabbits. An antibody response was elicited not only by the full-length synthetic VPg peptide, but also by a synthetic 14-amino acid carboxy-terminal peptide. All antisera reacted with virus-specific proteins from HeLa cells infected with poliovirus. Three of these proteins have previously been implicated by others as precursors of VPg. No free cytoplasmic VPg could be detected, and the antibodies did not react with radiolabeled proteins from uninfected cells.
脊髓灰质炎病毒的基因组连接蛋白（VPg）已被化学合成，与牛血清白蛋白载体偶联后注射到兔子体内。不仅全长合成VPg肽引发了抗体反应，一种合成的14个氨基酸的羧基末端肽也引发了抗体反应。所有抗血清都与感染脊髓灰质炎病毒的HeLa细胞中的病毒特异性蛋白发生反应。其他研究人员先前已将其中三种蛋白认定为VPg的前体。未检测到游离的细胞质VPg，且抗体不与未感染细胞的放射性标记蛋白发生反应。
