Ikehara Y, Oda K, Rosenfeld M G, Bar-Nun S, Kreibich G
J Biochem. 1981 Dec;90(6):1833-6. doi: 10.1093/oxfordjournals.jbchem.a133664.
The biosynthesis of alpha 2 u-globulin, the major protein in rat urine, was studied in a cell-free system programmed with messenger RNA isolated from rat liver. Analysis of the primary translation product by SDS-polyacrylamide gel electrophoresis showed a molecular weight of approximatley 22,000 daltons as compared to 20,000 daltons for the mature form. When translation was carried out in the presence of dog pancreas microsomes, alpha 2u-globulin was cotranslationally processed and sequestered in the microsomal lumen. Automated sequencing to mature alpha 2u-globulin and the labeled precursor disclosed the presence of 19 additional amino acids at the amino-terminal end of the primary translation product of the urinary alpha 2u-globulin. The partial amino acid sequence of this extension was determined.
对大鼠尿液中的主要蛋白质α2u-球蛋白的生物合成，在一个用从大鼠肝脏分离的信使核糖核酸编程的无细胞系统中进行了研究。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对初级翻译产物的分析显示，其分子量约为22,000道尔顿，而成熟形式的分子量为20,000道尔顿。当在狗胰腺微粒体存在的情况下进行翻译时，α2u-球蛋白会进行共翻译加工并被隔离在微粒体腔中。对成熟的α2u-球蛋白和标记前体进行自动测序，揭示了尿α2u-球蛋白初级翻译产物氨基末端存在另外19个氨基酸。确定了这个延伸部分的部分氨基酸序列。
