角蛋白在PtK1细胞骨架上的组装：中间丝组织中心的证据。
Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center.
作者信息
Eckert B S, Daley R A, Parysek L M
出版信息
J Cell Biol. 1982 Feb;92(2):575-8. doi: 10.1083/jcb.92.2.575.
Abstract
Purified keratin, solubilized in 8 M of urea, was added to Triton X-100-extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cells and frequently form bridges between adjacent cells. Electron microscopy shows that keratin filaments associate closely with the surface of the nucleus. We suggest that the site of association between keratin and the nucleus may represent an intermediate filament organizing center.
摘要
将溶解于8M尿素中的纯化角蛋白添加到含有5mM PIPES缓冲液的经Triton X-100处理的PtK1细胞中。这种缓冲液条件诱导角蛋白丝组装，这些角蛋白丝似乎与经处理细胞的细胞核相关联。这些角蛋白纤维延伸超出细胞的原始边缘，并经常在相邻细胞之间形成桥。电子显微镜显示角蛋白丝与细胞核表面紧密相关。我们认为角蛋白与细胞核之间的关联位点可能代表一个中间丝组织中心。
相似文献
1
Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center.角蛋白在PtK1细胞骨架上的组装：中间丝组织中心的证据。
J Cell Biol. 1982 Feb;92(2):575-8. doi: 10.1083/jcb.92.2.575.
2
Dynamics of keratin filaments and the intermediate filament distribution center during shape change in PtK1 cells.PtK1细胞形态变化过程中角蛋白丝和中间丝分布中心的动态变化
Cell Motil. 1984;4(3):169-81. doi: 10.1002/cm.970040303.
3
Modulation of keratin intermediate filament distribution in vivo by induced changes in cyclic AMP-dependent phosphorylation.通过诱导环磷酸腺苷（cAMP）依赖性磷酸化的变化在体内调节角蛋白中间丝的分布。
Cell Motil Cytoskeleton. 1990;17(4):291-300. doi: 10.1002/cm.970170404.
4
Localization of the centriole and keratin intermediate filaments in PtK1 cells by double immunofluorescence.通过双重免疫荧光法对PtK1细胞中的中心粒和角蛋白中间丝进行定位。
Cell Motil. 1984;4(4):241-7. doi: 10.1002/cm.970040403.
5
Epidermal alpha-keratin is neutral-buffer-soluble and forms intermediate filaments under physiological conditions in vitro.表皮α-角蛋白可溶于中性缓冲液，并在体外生理条件下形成中间丝。
Biochim Biophys Acta. 1987 Sep 2;915(1):125-31. doi: 10.1016/0167-4838(87)90132-4.
6
Characterization of early assembly intermediates of recombinant human keratins.重组人角蛋白早期组装中间体的表征
J Struct Biol. 2002 Jan-Feb;137(1-2):82-96. doi: 10.1006/jsbi.2002.4466.
7
A novel mechanism of keratin cytoskeleton organization through casein kinase Iα and FAM83H in colorectal cancer.一种通过酪蛋白激酶 Iα 和 FAM83H 调节结直肠癌角蛋白细胞骨架组织的新机制。
J Cell Sci. 2013 Oct 15;126(Pt 20):4721-31. doi: 10.1242/jcs.129684. Epub 2013 Jul 31.
8
De novo synthesis and specific assembly of keratin filaments in nonepithelial cells after microinjection of mRNA for epidermal keratin.在显微注射表皮角蛋白mRNA后非上皮细胞中角蛋白丝的从头合成和特异性组装。
Cell. 1983 Apr;32(4):1125-37. doi: 10.1016/0092-8674(83)90296-9.
9
10
Effects of Plectin Depletion on Keratin Network Dynamics and Organization.网蛋白缺失对角蛋白网络动力学及组织的影响。
PLoS One. 2016 Mar 23;11(3):e0149106. doi: 10.1371/journal.pone.0149106. eCollection 2016.
引用本文的文献
1
Identification of novel principles of keratin filament network turnover in living cells.活细胞中角蛋白丝网络周转新原理的鉴定。
Mol Biol Cell. 2004 May;15(5):2436-48. doi: 10.1091/mbc.e03-09-0707. Epub 2004 Mar 5.
2
Vimentin filaments in spreading, randomly locomoting, and f-met-leu-phe-treated neutrophils.
Cell Tissue Res. 1984;235(3):575-81. doi: 10.1007/BF00226955.
3
Intermediate filaments in monkey kidney TC7 cells: focal centers and interrelationship with other cytoskeletal systems.猴肾TC7细胞中的中间丝：焦点中心及其与其他细胞骨架系统的相互关系。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1117-21. doi: 10.1073/pnas.81.4.1117.
4
Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells.完整的诺维科夫肝癌细胞中细胞角蛋白p39与DNA的关联
Proc Natl Acad Sci U S A. 1984 Jan;81(2):419-23. doi: 10.1073/pnas.81.2.419.
5
Dynamic aspects of intermediate filament networks in BHK-21 cells.BHK - 21细胞中中间丝网络的动态特性
Proc Natl Acad Sci U S A. 1989 Jan;86(2):549-53. doi: 10.1073/pnas.86.2.549.
6
Expression of mutant keratin cDNAs in epithelial cells reveals possible mechanisms for initiation and assembly of intermediate filaments.突变角蛋白cDNA在上皮细胞中的表达揭示了中间丝起始和组装的可能机制。
J Cell Biol. 1989 Apr;108(4):1477-93. doi: 10.1083/jcb.108.4.1477.
7
The expression of mutant epidermal keratin cDNAs transfected in simple epithelial and squamous cell carcinoma lines.在简单上皮细胞和鳞状细胞癌系中转染的突变型表皮角蛋白cDNA的表达。
J Cell Biol. 1987 Aug;105(2):791-806. doi: 10.1083/jcb.105.2.791.
8
Monoclonal antibody analysis of keratin expression in the central nervous system.
Proc Natl Acad Sci U S A. 1987 May;84(10):3482-5. doi: 10.1073/pnas.84.10.3482.
9
Filensin: a new vimentin-binding, polymerization-competent, and membrane-associated protein of the lens fiber cell.丝状晶蛋白：一种新的与波形蛋白结合、具有聚合能力且与晶状体纤维细胞膜相关的蛋白质。
J Cell Biol. 1991 Oct;115(2):397-410. doi: 10.1083/jcb.115.2.397.
10
Intermediate filaments formed de novo from tail-less cytokeratins in the cytoplasm and in the nucleus.由无尾细胞角蛋白在细胞质和细胞核中重新形成的中间丝。
J Cell Biol. 1991 Dec;115(5):1293-307. doi: 10.1083/jcb.115.5.1293.
本文引用的文献
1
Fine structure of an organelle associated with the nucleus and cytoplasmic microtubules in the cellular slime mould Polysphondylium violaceum.与细胞黏菌紫多头绒泡菌中的细胞核和细胞质微管相关的一种细胞器的精细结构。
J Cell Sci. 1975 Jul;18(2):315-26. doi: 10.1242/jcs.18.2.315.
2
Self-assembly of bovine epidermal keratin filaments in vitro.牛表皮角蛋白丝在体外的自组装
J Mol Biol. 1976 Dec 15;108(3):547-67. doi: 10.1016/s0022-2836(76)80136-2.
3
Microtubules in cultured cells; indirect immunofluorescent staining with tubulin antibody.培养细胞中的微管；用微管蛋白抗体进行间接免疫荧光染色。
Int Rev Cytol. 1980;63:59-95. doi: 10.1016/s0074-7696(08)61757-x.
4
HeLa cells contain intermediate-sized filaments of the prekeratin type.
Exp Cell Res. 1979 Jan;118(1):95-109. doi: 10.1016/0014-4827(79)90587-1.
Zotero 插件