角蛋白在PtK1细胞骨架上的组装：中间丝组织中心的证据。
Assembly of keratin onto PtK1 cytoskeletons: evidence for an intermediate filament organizing center.
作者信息
Eckert B S, Daley R A, Parysek L M
出版信息
J Cell Biol. 1982 Feb;92(2):575-8. doi: 10.1083/jcb.92.2.575.
Abstract
Purified keratin, solubilized in 8 M of urea, was added to Triton X-100-extracted PtK1 cells in 5 mM PIPES buffer. The buffer conditions induced assembly of keratin filaments which appear to associate with nuclei of extracted cells. These keratin fibers extend beyond the original margin of the cells and frequently form bridges between adjacent cells. Electron microscopy shows that keratin filaments associate closely with the surface of the nucleus. We suggest that the site of association between keratin and the nucleus may represent an intermediate filament organizing center.
摘要
将溶解于8M尿素中的纯化角蛋白添加到含有5mM PIPES缓冲液的经Triton X-100处理的PtK1细胞中。这种缓冲液条件诱导角蛋白丝组装，这些角蛋白丝似乎与经处理细胞的细胞核相关联。这些角蛋白纤维延伸超出细胞的原始边缘，并经常在相邻细胞之间形成桥。电子显微镜显示角蛋白丝与细胞核表面紧密相关。我们认为角蛋白与细胞核之间的关联位点可能代表一个中间丝组织中心。
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