Dickinson H G
J Cell Sci. 1981 Dec;52:223-41. doi: 10.1242/jcs.52.1.223.
The unusual content of the plastids characteristic of male meiosis and microsporogenesis in Lilium henryi has been investigated using enzymic digestion of material prepared for electron microscopy. Apart from membranous tubules and osmiophilic droplets, commonly regarded as normal constituents of the stroma of undifferentiated plastids, these organelles contained a single conspicuous association between membrane and particulate material and wefts of filaments. Enzymic digestion revealed the membrane-particle association (MPA) to contain RNA and some protein, and the filaments to be entirely proteinaceous. The use of DNase on this material proved both unreliable and, on occasions, unspecific, so no new information has emerged as to the disposition of DNA within these organelles. Many of the plastids divide immediately after meiosis and the MPAs normally divide in step with the organelles. The activities of the MPA and those of the filaments are discussed in terms of changes in the organelle population as a whole, and also in consideration of the fundamental events occurring elsewhere in the cells during the alternation from the diploid sporophyte to the haploid gametophyte.
利用电子显微镜制备材料的酶解方法，对岷江百合雄性减数分裂和小孢子发生过程中质体的异常内容物进行了研究。除了膜性小管和嗜锇滴（通常被视为未分化质体基质的正常成分）外，这些细胞器在膜与颗粒物质以及丝状束之间存在单一明显的关联。酶解显示膜 - 颗粒关联物（MPA）含有RNA和一些蛋白质，而丝状束完全由蛋白质组成。在这种材料上使用DNase证明既不可靠，有时也不具有特异性，因此关于这些细胞器内DNA的分布没有新的信息出现。许多质体在减数分裂后立即分裂，MPA通常与细胞器同步分裂。从整个细胞器群体的变化角度，以及考虑从二倍体孢子体到单倍体配子体转变过程中细胞其他部位发生的基本事件，对MPA和丝状束的活性进行了讨论。
