Auchincloss H, Ozato K, Sachs D H
J Immunol. 1982 Apr;128(4):1584-9.
20-10-5S is a monoclonal antibody produced by the fusion of C3H anti-C3H.SW splenocytes with the SP2/0 cell line. The antibody appears to react with Thy-1 determinants by several criteria including cytotoxicity patterns, functional assays, genetic analyses, and competitive binding experiments. However, the antibody and the determinants it detects are unusual in that: 1) 20-10-5S is autoreactive; 2) the antibody shows allospecificity for Thy-1.2 vs Thy-1.1 antigens only on peripheral lymphocytes and not on thymocytes; and 3) the antibody reacts only with determinants on murine T cells and not with antigens on brain tissue or on rat thymocytes. It therefore seems that 20-10-5S reacts with murine T cell-specific Thy-1 determinants that are lost or modified during maturation of the cells on which they are expressed.
20-10-5S是一种单克隆抗体，由C3H抗C3H.SW脾细胞与SP2/0细胞系融合产生。根据细胞毒性模式、功能测定、遗传分析和竞争性结合实验等多项标准，该抗体似乎与Thy-1决定簇发生反应。然而，该抗体及其检测到的决定簇具有以下不同寻常之处：1）20-10-5S具有自身反应性；2）该抗体仅在外周淋巴细胞而非胸腺细胞上对Thy-1.2与Thy-1.1抗原表现出同种特异性；3）该抗体仅与小鼠T细胞上的决定簇发生反应，而不与脑组织或大鼠胸腺细胞上的抗原发生反应。因此，20-10-5S似乎与小鼠T细胞特异性的Thy-1决定簇发生反应，这些决定簇在其表达的细胞成熟过程中会丢失或发生修饰。
