Andreeff M, Darzynkiewicz Z
Clin Bull. 1981;11(3):120-30.
The application of flow cytometry to hematology is emerging as an important new technique in experimental and clinical leukemic research. In addition to the described methods to determine DNA, RNA, and esterase content and the stability of DNA in situ, quantitation of immunofluorescence using antibody techniques has greatly elucidated the hematopoietic cell system. Hence, flow cytometry provides new perspective for determining the origin, diagnosis, classification, staging and treatment scheduling of normal hematopoietic and malignant cell populations.
流式细胞术在血液学中的应用正成为实验性和临床白血病研究中的一项重要新技术。除了已描述的用于测定DNA、RNA和酯酶含量以及原位DNA稳定性的方法外，利用抗体技术对免疫荧光进行定量分析极大地阐明了造血细胞系统。因此，流式细胞术为确定正常造血细胞和恶性细胞群体的起源、诊断、分类、分期及治疗方案提供了新的视角。
