细胞特异性染色体蛋白的固相放射免疫测定
Solid-phase radioimmunoassay of cell specific chromosomal proteins.
作者信息
McCormack M, Dunn J H, Campbell A M
出版信息
J Immunol Methods. 1982 Mar 12;49(2):151-7. doi: 10.1016/0022-1759(82)90273-3.
PMID:6175702
Abstract
A solid-phase radioimmunoassay has been developed for the detection of cell specific chromosomal proteins which require the presence of DNA for their immunoreactivity. The assay involves the binding of chromatin particles sonicated under highly controlled conditions to PVC plates followed by first and second antibody with extensive detergent washing. The sensitivity and specificity of the assay system compares favourably with the complement fixation technique.
摘要
已开发出一种固相放射免疫测定法，用于检测细胞特异性染色体蛋白，这些蛋白的免疫反应性需要DNA的存在。该测定法包括在高度受控条件下将超声处理的染色质颗粒与PVC板结合，然后依次加入第一抗体和第二抗体，并进行大量的去污剂洗涤。该测定系统的灵敏度和特异性与补体结合技术相比具有优势。
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