Jakob A, Becker J, Isler N, Diem S
J Cardiovasc Pharmacol. 1982;4 Suppl 1:S68-71. doi: 10.1097/00005344-198200041-00014.
Ethacrynic acid (ECA) was used to study the relationship between ion transport and alpha-adrenergic activation of glycogenolysis in perfused rat livers. ECA alone enhanced glycogenolysis and produced a massive loss of K+ from the liver. These effects were partially blocked by dithioerythritol. ECA suppressed the metabolic responses to phenylephrine and the alpha-adrenergic redistribution of ions. Ouabain-sensitive and ouabain-insensitive ATPase of isolated liver plasma membranes were inhibited and binding of [3H]epinephrine to alpha-adrenergic sites was decreased by ECA. It is concluded that in liver ECA acts at three different sites by blocking SH groups: active ion transport across the plasma membrane, alpha-adrenoreceptors, and phosphorylase phosphatase.
依他尼酸（ECA）被用于研究灌注大鼠肝脏中离子转运与糖原分解的α-肾上腺素能激活之间的关系。单独使用ECA可增强糖原分解，并导致肝脏大量钾离子流失。这些作用被二硫苏糖醇部分阻断。ECA抑制了对去氧肾上腺素的代谢反应以及离子的α-肾上腺素能重新分布。ECA抑制了分离的肝细胞膜上对哇巴因敏感和不敏感的ATP酶，并且降低了[3H]肾上腺素与α-肾上腺素能位点的结合。得出的结论是，在肝脏中，ECA通过阻断巯基作用于三个不同位点：跨质膜的主动离子转运、α-肾上腺素受体和磷酸化酶磷酸酶。
