Dillwith J W, Lewis R A
Arch Environ Contam Toxicol. 1982;11(1):41-6. doi: 10.1007/BF01055184.
The growth of E. coli in broth cultures was inhibited in a concentration dependent manner by the addition of captan. Treated cells showed no evidence of lysis and maintained a viable character. Following a single exposure to 500 microM captan, cells exhibited a lag period of approximately six hr and then grew to maximum density. During the period of arrested growth, captan apparently inhibited the synthesis of RNA as measured by the incorporation of tritiated uridine into acid-insoluble material. This apparent inhibition was due in part to a captan mediated decrease in the uptake of uridine from the media into the soluble nucleotide pool. By means of RNA isolation, polyacrylamide gel electrophoresis, and RNA quantitation, it was demonstrated that captan caused a selective loss of large (16 and 23 S) ribosomal RNA's. However, cellular concentrations of transfer RNA and 5S ribosomal RNA were unaffected by captan treatment.
在肉汤培养物中，克菌丹的添加以浓度依赖的方式抑制了大肠杆菌的生长。经处理的细胞没有裂解迹象，并保持存活特性。单次暴露于500微摩尔克菌丹后，细胞表现出约6小时的延迟期，然后生长至最大密度。在生长停滞期间，通过将氚标记的尿苷掺入酸不溶性物质来测量，克菌丹明显抑制了RNA的合成。这种明显的抑制部分是由于克菌丹介导的尿苷从培养基进入可溶性核苷酸池的摄取减少。通过RNA分离、聚丙烯酰胺凝胶电泳和RNA定量分析，证明克菌丹导致了大（16和23 S）核糖体RNA的选择性损失。然而，转运RNA和5S核糖体RNA的细胞浓度不受克菌丹处理的影响。
