Dent P B, Liao S K, McCulloch P B, Stone B R, Singal D P
Cancer. 1982 May 15;49(10):2043-8. doi: 10.1002/1097-0142(19820515)49:10<2043::aid-cncr2820491017>3.0.co;2-s.
While patients with melanoma are known to produce antibodies against melanoma cells, the tumor specificity of these reactions has not been well documented. Using the sensitive mixed hemadsorption assay we have identified antibody against one or more of nine different cultured melanoma cell lines in only nine of 48 patients with melanoma. Reactivity against melanoma cell lines was seen only in females, 9/27 versus 0/21 males. The strongest melanoma reactivity was seen in sera which also contained lymphocytotoxic antibody. The reactivity was not melanoma specific because it could be removed by absorption either with pooled platelets, nonmelanoma tumor cells or in two cases, by both. We conclude that the occurrence of specific antimelanoma antibodies against common or shared surface melanoma associated antigens is an uncommon event in melanoma patients not receiving specific active immunotherapy. The clinical significance of the observed reactivity and whether it is directed against cancer associated determinants, fetal antigens, or normal tissue or histocompatibility antigens requires further study.
虽然已知黑色素瘤患者会产生针对黑色素瘤细胞的抗体，但这些反应的肿瘤特异性尚未得到充分记录。使用灵敏的混合血细胞吸附试验，我们在48例黑色素瘤患者中仅9例发现了针对9种不同培养黑色素瘤细胞系中一种或多种的抗体。仅在女性中观察到对黑色素瘤细胞系的反应性，27例女性中有9例，而21例男性中为0例。在也含有淋巴细胞毒性抗体的血清中观察到最强的黑色素瘤反应性。这种反应性并非黑色素瘤特异性的，因为它可以通过与混合血小板、非黑色素瘤肿瘤细胞吸附去除，在两例中，两种方法均可去除。我们得出结论，在未接受特异性主动免疫治疗的黑色素瘤患者中，针对常见或共享的表面黑色素瘤相关抗原产生特异性抗黑色素瘤抗体是一种罕见事件。观察到的反应性的临床意义以及它是否针对癌症相关决定簇、胎儿抗原、正常组织或组织相容性抗原，需要进一步研究。
