Haroske G, Dimmer V
Exp Pathol. 1982;21(1):21-35.
Non-labelled lymphocytes in peripheral blood smears and in smears of purified lymphocyte suspensions were differentiated in T- and non-T-cells (controlled by E-rosetting assay and surface immunoglobulin detection) by automatic image processing at the light microscopic level. By various classification methods it was found that these lymphocyte subpopulations differ in several visual and subvisual morphologic features. The morphologic differences between subpopulations were superimposed by individual deviations as well as by influences from the preparation. Single features were strongly affected by exogenic factors. Most of the different features are comparable with fine structural differences, as previously reported.
通过光学显微镜水平的自动图像处理，对外周血涂片和纯化淋巴细胞悬液涂片中的未标记淋巴细胞，根据E-玫瑰花结试验和表面免疫球蛋白检测结果，区分出T细胞和非T细胞。通过各种分类方法发现，这些淋巴细胞亚群在几个视觉和亚视觉形态特征上存在差异。亚群之间的形态学差异因个体偏差以及制片的影响而叠加。单个特征受外源性因素的强烈影响。如先前报道的那样，大多数不同特征与细微结构差异具有可比性。
