显微注射小鼠肝脏mRNA后从蛙卵母细胞输出的白蛋白的性质

Nature of albumin exported from the frog oocyte following microinjection of mouse liver mRNA.

作者信息

Teraoka H, Arita H, Tamaki M, Fujii Y, Tanaka K

出版信息

J Biochem. 1982 Mar;91(3):933-8. doi: 10.1093/oxfordjournals.jbchem.a133782.

DOI:10.1093/oxfordjournals.jbchem.a133782

Abstract

Microinjections of mouse liver mRNA into Xenopus laevis oocytes induced efficient export of a polypeptide with an apparent Mr or 68,000 which was immunoprecipitable with anti-mouse albumin antibody. Analysis of the anti-albumin precipitate of the exported protein by two-dimensional gel electrophoresis showed that the electrophoretic behavior precisely coincided with that of authentic mouse serum albumin. This result indicates that the Xenopus oocyte may perform secretory processes similar or identical to those occurring in liver cells with respect to the processing of albumin.

摘要

将小鼠肝脏mRNA显微注射到非洲爪蟾卵母细胞中，诱导出一种表观分子量为68,000的多肽有效输出，该多肽可用抗小鼠白蛋白抗体进行免疫沉淀。通过二维凝胶电泳分析输出蛋白的抗白蛋白沉淀物，结果表明其电泳行为与 authentic小鼠血清白蛋白完全一致。这一结果表明，非洲爪蟾卵母细胞在白蛋白加工方面可能执行与肝细胞中相似或相同的分泌过程。