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心肌梗死中的宏观酶组织化学：辅酶、氰化物和吩嗪硫酸甲酯的应用

Macroscopic enzyme histochemistry in myocardial infarction: use of coenzyme, cyanide, and phenazine methosulphate.

作者信息

Derias N W, Adams C W

出版信息

J Clin Pathol. 1982 Apr;35(4):410-3. doi: 10.1136/jcp.35.4.410.

DOI:10.1136/jcp.35.4.410
PMID:6176598
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC497671/
Abstract

Transversely sectioned human heart slices, obtained at necropsy from normal subjects and from cases of recent myocardial infarction, were stained with the nitroblue tetrazolium (NBT) dehydrogenase macroreaction for the gross identification of recent myocardial infarction. The addition of nicotinamide adenine dinucleotide (NAD) to the incubating medium greatly improved the sensitivity of the method, while addition of cyanide caused just a modest improvement. Addition of the electron transfer mediator phenazine methosulphate (PMS) resulted in false non-selective staining and obscured areas of recent myocardial damage.

摘要

从正常受试者和近期心肌梗死病例尸检中获取的横向切片人类心脏组织，用硝基蓝四氮唑（NBT）脱氢酶宏观反应染色，以大体识别近期心肌梗死。在孵育培养基中添加烟酰胺腺嘌呤二核苷酸（NAD）可大大提高该方法的灵敏度，而添加氰化物仅带来适度改善。添加电子传递介质吩嗪硫酸甲酯（PMS）会导致假的非选择性染色，并掩盖近期心肌损伤区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/d64bb053331b/jclinpath00497-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/9da8953def3e/jclinpath00497-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/4b590bfeb3d8/jclinpath00497-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/d64bb053331b/jclinpath00497-0044-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/9da8953def3e/jclinpath00497-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/4b590bfeb3d8/jclinpath00497-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98f2/497671/d64bb053331b/jclinpath00497-0044-b.jpg

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Macroscopic enzyme histochemistry in myocardial infarction: use of coenzyme, cyanide, and phenazine methosulphate.心肌梗死中的宏观酶组织化学：辅酶、氰化物和吩嗪硫酸甲酯的应用
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引用本文的文献

1
Macroscopic enzyme histochemistry in myocardial infarction: artefactual nature of the creatine phosphokinase reaction.心肌梗死中的宏观酶组织化学：肌酸磷酸激酶反应的人为性质。
J Clin Pathol. 1982 Apr;35(4):407-9. doi: 10.1136/jcp.35.4.407.
2
Phenazine methosulphate and the nitroblue tetrazolium macroreaction for recent myocardial infarction.吩嗪硫酸甲酯与硝基蓝四氮唑用于近期心肌梗死的宏观反应
J Clin Pathol. 1984 Apr;37(4):477-8. doi: 10.1136/jcp.37.4.477.
3
The 'nothing dehydrogenase' reaction and the detection of ischaemic damage.

本文引用的文献

1
Macroscopic identification of early myocardial infarcts by alterations in dehydrogenase activity.通过脱氢酶活性改变对早期心肌梗死进行宏观鉴定。
Am J Pathol. 1963 Apr;42(4):379-405.
2
The value of the nitro-BT method in fresh myocardial infarction. Frequency and location of fresh myocardial infarction in a consecutive series of autopsies.
Am Heart J. 1973 May;85(5):611-9. doi: 10.1016/0002-8703(73)90166-x.
3
Nitroblue tetrazolium test: early gross detection of human myocardial infarcts.硝基蓝四氮唑试验：人类心肌梗死的早期大体检测
“无脱氢酶”反应与缺血性损伤的检测
Histochem J. 1989 Sep-Oct;21(9-10):565-73. doi: 10.1007/BF01753357.
4
Necropsy study of association between sudden death and cardiac enzymes.猝死与心肌酶之间关联的尸检研究
J Clin Pathol. 1992 Mar;45(3):217-20. doi: 10.1136/jcp.45.3.217.
Br J Exp Pathol. 1978 Jun;59(3):254-8.
4
The pathology of myocardial ischaemia.心肌缺血的病理学
J Clin Pathol Suppl (R Coll Pathol). 1977;11:45-52. doi: 10.1136/jcp.s3-11.1.45.
5
Pathology of acute ischemic myocardium. Special references to (I) evaluation of morphological methods for detection of early myocardial infarcts, and (II) lipid metabolism in infarcted myocardium.急性缺血性心肌的病理学。特别涉及（I）检测早期心肌梗死形态学方法的评估，以及（II）梗死心肌中的脂质代谢。
Acta Pathol Jpn. 1977 Sep;27(5):587-603. doi: 10.1111/j.1440-1827.1977.tb00179.x.