Borges L F, Sidman R L
J Neurosci. 1982 May;2(5):647-53. doi: 10.1523/JNEUROSCI.02-05-00647.1982.
The binding and axonal transport of six lectins were studied in the peripheral nervous system of adult mice by an immunocytochemical method. After injection into muscle and subcutaneous sites, lectins known to bind preferentially N-acetylglucosamine or mannose sugars were transported axonally to ventral horn and dorsal root ganglion neurons. Twelve to 96 hr postinjection, these lectins were bound at the injection site to neuromuscular junctions, muscle spindles, and cutaneous nerves. Lectins known to bind preferentially N-acetylgalactosamine or galactose sugars, by contrast, were transported only to dorsal root ganglion neurons. Except for Sophora japonica agglutinin, these lectins were bound at the injection site only to cutaneous nerves. These differences in axonal transport were seen also when the lectins were applied directly to the proximal end of a transected mixed nerve.
采用免疫细胞化学方法，研究了六种凝集素在成年小鼠外周神经系统中的结合及轴突运输情况。将凝集素注射到肌肉和皮下部位后，已知优先结合N-乙酰葡糖胺或甘露糖的凝集素会通过轴突运输至腹角和背根神经节神经元。注射后12至96小时，这些凝集素在注射部位与神经肌肉接头、肌梭和皮神经结合。相比之下，已知优先结合N-乙酰半乳糖胺或半乳糖的凝集素仅运输至背根神经节神经元。除了槐凝集素外，这些凝集素在注射部位仅与皮神经结合。当将凝集素直接应用于横断混合神经的近端时，也观察到了轴突运输的这些差异。
