Lloyd E J, Barnes D M, Skinner L G
J Steroid Biochem. 1982 Feb;16(2):239-44. doi: 10.1016/0022-4731(82)90172-8.
Two simple methods for the detection of specific oestradiol--17 beta protein in breast tumour tissue were investigated. Isoelectric focusing of receptor protein in polyacrylamide gel was examined as an alternative micromethod to dextran-coated charcoal receptor assay. In a series of 32 cytosols prepared from unselected breast tumour tissue, assay results agreed on the presence or absence of receptors in 97% of cases. Quantitative analysis of the positive results showed a correlation coefficient, r = 0.88. Isoelectric focusing gave significantly lower receptor concentrations than the dextran-coated charcoal method. Twenty-six Tru-Cut biopsy tumour specimens were analyzed for receptor by isoelectric focusing and the results were compared with those obtained by the dextran-coated charcoal method on the same tumour. In 88% of the specimens examined, both methods agreed as to the presence or absence of receptor protein. A correlation coefficient, r = 0.62, was calculated from the 15 positive results. There was no significant difference between the results from the two methods. Quantitative results were related to total protein concentration in the cytosol. Negative receptor status was defined as less than 5 fmol/mg cytosol protein for both methods.
研究了两种检测乳腺肿瘤组织中特异性雌二醇 - 17β蛋白的简单方法。研究了聚丙烯酰胺凝胶中受体蛋白的等电聚焦，作为葡聚糖包被活性炭受体测定法的替代微量方法。在一系列由未经选择的乳腺肿瘤组织制备的32种细胞溶胶中，97%的病例检测结果在受体存在与否上一致。对阳性结果的定量分析显示相关系数r = 0.88。等电聚焦得到的受体浓度明显低于葡聚糖包被活性炭法。通过等电聚焦分析了26个Tru - Cut活检肿瘤标本的受体，并将结果与同一肿瘤用葡聚糖包被活性炭法得到的结果进行比较。在88%的检测标本中，两种方法在受体蛋白存在与否上一致。从15个阳性结果计算出相关系数r = 0.62。两种方法的结果无显著差异。定量结果与细胞溶胶中的总蛋白浓度相关。两种方法均将受体阴性状态定义为低于5 fmol/mg细胞溶胶蛋白。
