通过单克隆抗-HBs抗体放射免疫测定法在澳大利亚原住民人群中发现先前未检测到的乙型肝炎病毒决定簇

Demonstration of previously undetected hepatitis B viral determinants in an Australian Aboriginal population by monoclonal anti-hbs antibody radioimmunoassays.

作者信息

Wands J R, Marciniak R A, Isselbacher K J, Varghese M, Don G, Halliday J W, Powell L W

出版信息

Lancet. 1982 May 1;1(8279):977-80. doi: 10.1016/s0140-6736(82)91988-2.

DOI:10.1016/s0140-6736(82)91988-2

Abstract

High-affinity IgM and IgG monoclonal antibodies (anti-HBs) against hepatitis B surface antigen (HBsAg) determinants were used to study a confined Australian Aboriginal population, 51% of which showed evidence of exposure to hepatitis B virus (HBV). A conventional radioimmunoassay which uses polyvalent anti-HBs antisera indicated that 4.4% of the subjects were positive for HBsAg; a monoclonal IgM anti-HBs radioimmunoassay detected all these HBsAg-positive samples and showed enhanced binding activity in a further 5.4% of subjects. Detailed analysis of this binding activity in serum by different IgG and IgM monoclonal anti-HBs antibodies demonstrated additional HBsAg-associated determinants as well as remarkable homogeneity of the determinants in this population. It was concluded that there are HBV or HBV-like agents in this community not previously detected by conventional assays.

摘要

使用针对乙型肝炎表面抗原（HBsAg）决定簇的高亲和力IgM和IgG单克隆抗体（抗-HBs）对澳大利亚一个封闭的原住民群体进行研究，其中51%的人有接触乙型肝炎病毒（HBV）的证据。一种使用多价抗-HBs抗血清的传统放射免疫测定法表明，4.4%的受试者HBsAg呈阳性；一种单克隆IgM抗-HBs放射免疫测定法检测到了所有这些HBsAg阳性样本，并在另外5.4%的受试者中显示出增强的结合活性。通过不同的IgG和IgM单克隆抗-HBs抗体对血清中这种结合活性进行详细分析，发现了额外的与HBsAg相关的决定簇，以及该群体中决定簇的显著同质性。得出的结论是，该社区存在传统检测方法以前未检测到的HBV或类HBV病原体。