Shafritz D A, Lieberman H M, Isselbacher K J, Wands J R
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5675-9. doi: 10.1073/pnas.79.18.5675.
High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays for the detection of HBsAg-associated determinants in serum. We now report that selected serum samples demonstrating monoclonal immunoreactive material also contain nucleic acid sequences complementary to hepatitis B virus (HBV) DNA by molecular hybridization analysis. In addition, monoclonal radioimmunoassays can detect viral determinants in HBsAg-anti-HBs immune complexes formed in anti-HBs excess; such determinants are undetectable by commercial radioimmunoassay. These and our previous studies suggest that there are HBV or "HBV-related" agents present in human serum that are detected by monoclonal antibodies but are not identified by conventional polyvalent antibodies.
我们最近研制出了对乙型肝炎表面抗原（HBsAg）具有高亲和力的单克隆IgM和IgG抗体（抗-HBs），并将其用于构建高灵敏度放射免疫分析法，以检测血清中与HBsAg相关的决定簇。我们现在报告，通过分子杂交分析，显示单克隆免疫反应性物质的选定血清样本还含有与乙型肝炎病毒（HBV）DNA互补的核酸序列。此外，单克隆放射免疫分析法可以检测在抗-HBs过量时形成的HBsAg-抗-HBs免疫复合物中的病毒决定簇；而商业放射免疫分析法无法检测到这些决定簇。这些以及我们之前的研究表明，人血清中存在被单克隆抗体检测到但未被传统多价抗体识别的HBV或“HBV相关”因子。
