Howell J N
Membr Biochem. 1982;4(3):235-45. doi: 10.3109/09687688209065433.
The multivalent anions, ATP and oxalate, present at 5mM concentrations in incubation mixtures with isolated sarcoplasmic reticulum (SR) preparations, reduce the binding of ruthenium red (RR) to the SR. When oxalate is omitted from the incubation mixture and ATP is used at reduced concentrations, it is possible to observe an inhibitory effect of RR on calcium uptake by the SR and on ATPase activity. However, this inhibition is only partial and it remains clear that calcium transport in the SR is much less susceptible to inhibition by RR than is calcium transport in mitochondria. The effect of multivalent anions in suppressing the effect of RR, a hexavalent cation, is probably due to the formation of soluble complexes with RR.
在与分离的肌浆网（SR）制剂的孵育混合物中，浓度为5mM的多价阴离子ATP和草酸盐会减少钌红（RR）与SR的结合。当孵育混合物中省略草酸盐且ATP以较低浓度使用时，有可能观察到RR对SR摄取钙以及对ATP酶活性的抑制作用。然而，这种抑制只是部分的，并且很明显，SR中的钙转运比线粒体中的钙转运对RR抑制的敏感性要低得多。多价阴离子抑制六价阳离子RR作用的效应可能是由于与RR形成了可溶性复合物。
