Mares V, Kovárů F, Kovárů H
Basic Appl Histochem. 1982;26(1):53-63.
Alpha-fetoprotein (AFP) and some other serum proteins have been studied in the developing brain of rats and pigs using the indirect immunofluorescence technique. AFP is shown to be present in the ventricular ependyma, meningeal envelopes, the choroid plexus, blood vessel walls and in a wide scale of differentiating parenchymal cells ever since early embryonic ages of both species. In brain parenchyma the content of AFP is low in immature germinative cells; in both species it starts increasing in postmigratory neuroblasts and reaches a peak at the time of accelerated nerve cell differentiation. In rats, the amount of AFP is highest towards the end of the first postnatal week; then it starts decreasing and disappears towards the end of the 3rd week. In both species AFP is localized in the cytoplasm of nerve cell perikarya and their differentiating processes. Higher concentration of this protein has often been observed at the axonal pole of the cytoplasm of differentiating pyramidal neurons. Immunoglobulin G has been found in non-parenchymal structures, and small amounts also in parenchymal cells of embryonic and early postnatal rats following a pattern of cell-and-tissue distribution similar to that of AFP. In pigs, a low amount of albumin has been shown in differentiating leptomeninges. These data suggest uptake of AFP, and some other serum proteins, from the cerebrospinal fluid into cells of the immature rat and pig brain and its increase (or higher binding) in differentiating neurons.
利用间接免疫荧光技术，对大鼠和猪发育中的大脑中的甲胎蛋白（AFP）及其他一些血清蛋白进行了研究。结果显示，自这两个物种的胚胎早期起，AFP就存在于心室室管膜、脑膜被膜、脉络丛、血管壁以及广泛的分化实质细胞中。在脑实质中，未成熟的生发细胞内AFP含量较低；在这两个物种中，AFP在迁移后的神经母细胞中开始增加，并在神经细胞加速分化时达到峰值。在大鼠中，AFP含量在出生后第一周结束时最高；然后开始下降，并在第三周结束时消失。在这两个物种中，AFP都定位于神经细胞胞体及其分化过程的细胞质中。在分化的锥体神经元细胞质的轴突端，经常观察到这种蛋白质的浓度较高。在非实质结构中发现了免疫球蛋白G，在胚胎期和出生后早期的大鼠实质细胞中也有少量存在，其细胞和组织分布模式与AFP相似。在猪中，已显示在分化的软脑膜中有少量白蛋白。这些数据表明，AFP及其他一些血清蛋白可从脑脊液被摄取到未成熟大鼠和猪脑的细胞中，并在分化的神经元中增加（或结合更多）。
