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用辣根过氧化物酶(HRP)逆行运输和4,6-二脒基-2-苯基吲哚(DAPI)鉴定的腰段节前神经元的不同群体。

Separate populations of lumbar preganglionic neurons identified with the retrograde transport of horseradish peroxidase (HRP) and 4,6-diamidino-2-phenylindole (DAPI).

作者信息

Hancock M B

出版信息

J Auton Nerv Syst. 1982 Mar;5(2):135-43. doi: 10.1016/0165-1838(82)90034-0.

DOI:10.1016/0165-1838(82)90034-0
PMID:6177726
Abstract

Horseradish peroxidase (HRP) was applied to the central cut end of the intermesenteric trunk or the caudal lumbar sympathetic trunk in golden hamsters. Preganglionic cells labeled from the intermesenteric trunk were located in the intermediolateral cell column, bilaterally, and in the dorsal commissural nucleus. The segmental distributions of the cells in these two nuclear groups were similar. Preganglionic neurons labeled from the lumbar sympathetic trunk were located mainly in the ipsilateral intermediolateral cell column. The simultaneous application of HRP to one trunk and 4,6-diamidino-2-phenylindole (DAPI) to the other delineated two separate preganglionic cell populations. Cells labeled from the two trunks were distributed as above. HRP-filled cells and DAPI-filled cells were intermixed in the intermediolateral cell column ipsilateral to the backfilled sympathetic trunk. No double labeled cells were present. The results show that the dorsal commissural nucleus in the lumbar cord preferentially innervates prevertebral ganglia, that lumbar paravertebral ganglia are innervated by cells located mainly in the ipsilateral intermediolateral cell column, and that paravertebral and prevertebral ganglia are innervated by non-overlapping sets of lumbar preganglionic neurons.

摘要

将辣根过氧化物酶(HRP)应用于金黄仓鼠肠系膜间干或尾侧腰交感干的中央切断端。从肠系膜间干标记的节前细胞双侧位于中间外侧细胞柱和背连合核中。这两个核团中细胞的节段分布相似。从腰交感干标记的节前神经元主要位于同侧的中间外侧细胞柱。同时将HRP应用于一个干,将4,6-二脒基-2-苯基吲哚(DAPI)应用于另一个干,可描绘出两个独立的节前细胞群。从两个干标记的细胞分布如上所述。在逆向填充的交感干同侧的中间外侧细胞柱中,HRP填充的细胞和DAPI填充的细胞相互混合。不存在双标记细胞。结果表明,腰髓中的背连合核优先支配椎前神经节,腰旁神经节主要由位于同侧中间外侧细胞柱的细胞支配,并且旁神经节和椎前神经节由不重叠的腰节前神经元支配。

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