Emekli N B, Ulutin O N
Bibl Haematol. 1977;44:15-20. doi: 10.1159/000402146.
Autoprothrombin II-A anticoagulant was isolated from bovine prothrombin. Purified prothrombin was applied to DEAE-cellulose chromatography after incubation with thrombin. Four protein peaks were obtained where the third peak corresponded to the anti-coagulant effect. The fractions under the third peak were pooled together and the anticoagulant effect was evaluated with different methods. From 25,470 +/- 2,800 U of prothrombin, 5,800 +/- 1,400 U of inhibitor were obtained. The inhibitor was found to be most effective at pH 7.2--7.8. In vitro, the inhibitor inhibited the thrombin time and the plasma clotting time highly significantly but had no effect on euglobulin lysis time and fibrin plates. In vivo, when injected into rabbits, the inhibitor effect was also significant on the same tests. The autoprothombin II-A anticoagulant had a protective effect on DIC formation with rabbit brain thromboplastin administration. This protective effect was found to be statistically significant.
自凝血酶原II-A抗凝剂是从牛凝血酶原中分离出来的。纯化后的凝血酶原在与凝血酶孵育后应用于DEAE-纤维素色谱法。得到了四个蛋白峰，其中第三个峰对应抗凝作用。合并第三个峰下的组分，并用不同方法评估其抗凝效果。从25,470±2,800单位的凝血酶原中获得了5,800±1,400单位的抑制剂。发现该抑制剂在pH 7.2-7.8时最有效。在体外，该抑制剂显著抑制凝血酶时间和血浆凝固时间，但对优球蛋白溶解时间和纤维蛋白平板无影响。在体内，当注入兔子体内时，在相同试验中该抑制剂的作用也很显著。自凝血酶原II-A抗凝剂对给予兔脑凝血活酶诱导的弥散性血管内凝血（DIC）形成具有保护作用。发现这种保护作用具有统计学意义。
