Koo G C, Peppard J R, Hatzfeld A
J Immunol. 1982 Aug;129(2):867-71.
Using anti-Nk-1.1 serum, the alloantiserum specific for murine natural killer (NK) cells, we followed the ontogenetic development of Nk-1+ cells in fetal thymus, liver, and spleen. A transient population of Nk-1+ cells in fetal thymus was observed on day 14 but not on day 16 of gestation. On day 16 of gestation, Nk-1+ cells were detected only in liver and spleen. The proportion of Nk-1+ cells in spleen remained high (20 to 30%) at birth and persisted until 2 to 3 wk old. The Nk-1+ cells in "baby" (1 to 2 wk old) spleen bound to YAC cells but failed to lyse them in 51Cr-release assay. Upon induction with interferon (IF), the proportion of Nk-1+ cells increased, but the lytic activity remained low, suggesting that the "baby" NK-1+ cells are immature in lytic function. In old mice (12 to 14 mo), Nk-1+ cells were also detectable, even though NK activities were lower compared with those of the young adult (6 to 8 wk old) mice. The Nk-1+ cells of old mice were readily induced by IF to exhibit activities, and the induced NK cells were Nk-1+. We have thus established Nk-1.1 antigen as an early hemopoietic differentiation antigen. Splenic Nk-1- cells could be induce by IF to become NK-1+ cells, which could be inactive or active in NK assays, dependent on the age of the mice.
我们使用抗Nk-1.1血清（一种对小鼠自然杀伤（NK）细胞具有特异性的同种异体抗血清），追踪了胎儿胸腺、肝脏和脾脏中Nk-1+细胞的个体发生发育情况。在妊娠第14天观察到胎儿胸腺中有短暂的Nk-1+细胞群体，但在妊娠第16天未观察到。在妊娠第16天，仅在肝脏和脾脏中检测到Nk-1+细胞。出生时脾脏中Nk-1+细胞的比例仍然很高（20%至30%），并持续到2至3周龄。“幼鼠”（1至2周龄）脾脏中的Nk-1+细胞与YAC细胞结合，但在51Cr释放试验中未能裂解它们。在用干扰素（IF）诱导后，Nk-1+细胞的比例增加，但裂解活性仍然很低，这表明“幼鼠”的NK-1+细胞在裂解功能上不成熟。在老年小鼠（12至14个月）中，即使与年轻成年（6至8周龄）小鼠相比NK活性较低，也能检测到Nk-1+细胞。老年小鼠的Nk-1+细胞很容易被IF诱导表现出活性，且诱导后的NK细胞为Nk-1+。因此，我们已将Nk-1.1抗原确立为一种早期造血分化抗原。脾脏中的Nk-1-细胞可被IF诱导成为NK-1+细胞，在NK试验中其可能无活性或有活性，这取决于小鼠的年龄。
