Taichman L B, Prokop C A
J Invest Dermatol. 1982 Jun;78(6):464-7. doi: 10.1111/1523-1747.ep12510153.
We have examined the synthesis of keratin and total protein during cellular maturation in cultured oral and epidermal keratinocytes. Keratinocytes were separated on the basis of maturational age on gradients of Ficoll 400. Analysis of selected gradient fractions revealed that during maturation in culture the amount of protein/cell increases from 100 to 900 pg and the apparent rate of protein synthesis increases about two-fold. However, the proportion of protein that is keratin remains constant throughout this process. The major keratin proteins (58K, 56K, and 46K) are synthesized at the same relative rates throughout maturation in vitro. Therefore, during terminal differentiation in culture, the keratinocyte forms an expanding cell in which the rate of protein synthesis increases while the relative proportion of keratin proteins and nonkeratin proteins remain the same.
我们研究了培养的口腔和表皮角质形成细胞在细胞成熟过程中角蛋白和总蛋白的合成情况。角质形成细胞根据在Ficoll 400梯度上的成熟年龄进行分离。对选定梯度组分的分析表明，在培养成熟过程中，蛋白质/细胞的量从100 pg增加到900 pg，蛋白质合成的表观速率增加约两倍。然而，在此过程中，角蛋白占蛋白质的比例保持恒定。主要角蛋白（58K、56K和46K）在体外成熟过程中以相同的相对速率合成。因此，在培养的终末分化过程中，角质形成细胞形成一个不断扩大的细胞，其中蛋白质合成速率增加，而角蛋白和非角蛋白的相对比例保持不变。
