血栓形成状态下凝血酶诱导的纤维蛋白原衍生物的检测。
Detection of thrombin-induced fibrinogen derivatives in thrombotic states.
作者信息
Heene D L, Matthias F R
出版信息
Bibl Haematol. 1977;44:161-8. doi: 10.1159/000402168.
Affinity chromatographic studies using insolubilized fibrinogen (fibrinogen-agarose) revealed that fibrin monomer present in plasma is selectively adsorbed to fibrinogen-agarose and may be quantitatively estimated following desorption. Analysis of plasma samples from patients with myocardial infarction, cirrhosis of the liver malignant diseases and DIC confirmed the presence of considerable amounts of fibrin monomer revealing concentrations between 3 and 15 mg/100 m1 plasma as compared to normal plasma (1.96 +/- 0.37 mg/100 m1, = 27). The method is suitable for the assessment of hypercoagulable states. Standard conditions for the procedure were evaluated using 3H-labelled fibrinogen and fibrin monomer.
使用不溶性纤维蛋白原（纤维蛋白原 - 琼脂糖）进行的亲和色谱研究表明，血浆中存在的纤维蛋白单体可选择性地吸附到纤维蛋白原 - 琼脂糖上，并且在解吸后可以进行定量估计。对心肌梗死、肝硬化、恶性疾病和弥散性血管内凝血患者的血浆样本分析证实，存在大量纤维蛋白单体，与正常血浆（1.96±0.37mg/100ml，n = 27）相比，其浓度在3至15mg/100ml血浆之间。该方法适用于评估高凝状态。使用³H标记的纤维蛋白原和纤维蛋白单体评估了该程序的标准条件。
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