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[大鼠小脑浦肯野细胞在不同功能状态下RNA定量变化的动态过程]

[Dynamics of quantitative RNA changes in the Purkinje cells of the rat cerebellum in various functional states].

作者信息

Mikeladze Z A

出版信息

Biull Eksp Biol Med. 1982 May;93(5):94-6.

PMID:6178454
Abstract

UV cytophotometry was used to study over time the effect of vestibular stimulation on RNA content in Purkinje's cells of the rat cerebellum nodulus. The animals placed in narrow boxes were rotated horizontally (60 r.p.m.) for an hour. To evaluate partially the effects of stress and hypoxia, the content of RNA was examined in the immobilized animals placed in the same boxes without rotation. Rhythmic quantitative changes in RNA in the cytoplasm and nucleus of Purkinje cells were observed upon vestibular stimulation of cerebellum function. In the nucleolus, the changes in the content of RNA were less pronounced. The periodic quantitative changes in the content of RNA in the cytoplasm, nucleus and nucleolus of Purkinje cells were statistically significant in the majority of the animals, but weakly pronounced.

摘要

采用紫外细胞光度法,长期研究前庭刺激对大鼠小脑蚓部浦肯野细胞中RNA含量的影响。将置于狭窄箱内的动物水平旋转(60转/分钟)一小时。为部分评估应激和缺氧的影响,检测了置于相同箱内但未旋转的固定动物的RNA含量。在前庭刺激小脑功能时,观察到浦肯野细胞胞质和细胞核中RNA有节律的定量变化。在核仁中,RNA含量的变化不太明显。在大多数动物中,浦肯野细胞胞质、细胞核和核仁中RNA含量的周期性定量变化具有统计学意义,但变化较弱。

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