[Cross reactivity of cytotoxic T-lymphocyte immune receptors immune to antigens of the H-2 complex studied by lymphocyte fractionation on target cell monolayers].

In vivo induced d anti-b spleen cytotoxic T-lymphocytes (CTL) display the cross lysis of H-2f and T-2a target cells (TC) which is about 4 to 6 per cent of the H-2b TC lysis as judged by comparison of CTL doses required for the same lysis. d anti-b CTL fractions cross-reacting (CR) to H-2f and H-2a TC are not identical and can be separated one from another by a selective adherence to the corresponding TC monolayer. The preliminary CTL absorption onto the syngeneic TC monolayer and the 3-step elution of the adherent CTL by pronase in two concentrations and EDTA gave rise to the optimizations of the CTL enrichment conditions and to the fractionation of CTL on the basis of stability of their contact with monolayer cells. The eluted CR d anti-b CTL fractions were found to destroy H-2b TC much stronger than TC of the CR strains from which CTL were eluted, to cross-react to the irrelevant CR antigen and to destroy H-2b TC as much as the CTL eluted from the H-2b monolayer. Besides, equalization and even invertion of the activity of CTL fractions was observed with respect to H-2b TC if CTL were eluted from CR strain monolayers. CTL receptors are suggested to be directed to the single CTL-determinant of an H-2 antigen and to be unable to seen particular serologically defined common specificities of the same antigen. The CTL cross reaction is supposed to be the variability in the unified CTL receptor complementarity (affinity); the less complementary (rigid) receptors being able to accommodate one of particular CR H-2 antigens.