在大肠杆菌K-12中克隆的噬菌体PM2片段的复制与表达。
Replication and expression of fragments of phage PM2 cloned in Escherichia coli K-12.
作者信息
Grzesiuk E, Rempoła B, Fikus M
出版信息
Acta Microbiol Pol. 1981;30(4):307-18.
PMID:6179390
Abstract
DNA from PM2 phage was cloned, as HindIII fragments and inserted into the pBR322 vector in E. coli cells. It was shown, that replication of recombined plasmids starts from the pBR322 origin. Transcription of recombinant plasmids in E. coli cells, as well as translation in minicells was demonstrated and attributed to pBR322 and/or PM2 DNA sequences.
摘要
将PM2噬菌体的DNA作为HindIII片段进行克隆，并插入大肠杆菌细胞中的pBR322载体。结果表明，重组质粒的复制起始于pBR322的原点。证实了重组质粒在大肠杆菌细胞中的转录以及在微细胞中的翻译，并归因于pBR322和/或PM2的DNA序列。
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