Tsoĭ T V, Gribanova L K, Kosheleva I A, Boronin A M
Genetika. 1988 Jun;24(6):980-92.
Rep-mob loci of naphthalene degradative plasmid pBS286 (IncP-9) have been cloned on the Escherichia coli vectors pUC19 and pUBR322. These loci confer to recombinant plasmids pBS952 and pBS953 the ability for effective mobilization by RP4 (IncP-1) and F plasmid, as well as constant maintenance in various gram-negative bacteria. Localization of cloned sequences in the restriction fragments of conservative part of the pBS286 genome was established. The data obtained correlate with the analysis of plasmids pBS950 and pBS951 which are spontaneous mini-derivatives of pBS286 and pBS292 (delta NPL1::Tn1/Tra+ Nah-) plasmids formed during transformation of E. coli HB101 cells. Plasmids pBS952 and pBS953 retain the incompatibility properties of parental IncP-9 replicon. These recombinant derivatives can be used for construction of bhr vectors with required properties and compatible with bhr vectors constructed on the basis of plasmids from the IncP-1 and IncP-4 groups.
萘降解性质粒pBS286（IncP-9）的复制-转移基因座已克隆到大肠杆菌载体pUC19和pUBR322上。这些基因座赋予重组质粒pBS952和pBS953被RP4（IncP-1）和F质粒有效转移的能力，以及在各种革兰氏阴性菌中稳定维持的能力。已确定克隆序列在pBS286基因组保守部分的限制片段中的定位。所获得的数据与对质粒pBS950和pBS951的分析相关，pBS950和pBS951是在大肠杆菌HB101细胞转化过程中形成的pBS286和pBS292（δNPL1::Tn1/Tra+ Nah-）质粒的自发微小衍生物。质粒pBS952和pBS953保留了亲本IncP-9复制子的不相容性。这些重组衍生物可用于构建具有所需特性且与基于IncP-1和IncP-4组质粒构建的bhr载体兼容的bhr载体。
