Nakao Y, Matsuda S, Fujita T, Watanabe S, Morikawa S, Saida T, Ito Y
Cancer Res. 1982 Sep;42(9):3843-50.
12-O-Tetradecanoylphorbol-13-acetate (TPA), a potent tumor promoter, induced phenotypic differentiation in the human thymic acute lymphocytic leukemia cell line, HPB-ALL. Within 30 min of seeding in the presence of TPA, the cells formed a smooth round shape. After a 7-day exposure to TPA, most of the cells became smaller and reminiscent of large or atypical lymphocytes. Electron microscopic analysis evidenced morphological differentiation in TPA-treated HPB-ALL cells. Thymic antigens stained with monoclonal antibody OKT6 were dramatically reduced while Leu2a-positive cells were increased in the TPA-treated HPB-ALL cells. However, OKT3-positive cells did not appear in these TPA-treated cells for up to 7 days. Upon TPA-induced phenotypic differentiation, the growth rate of cells was significantly inhibited, their ability to incorporate DNA and RNA via 3H-labeled precursors was reduced, their ability to bind sheep red blood cell rosettes was significantly increased, and the proportion of terminal deoxynucleotidyl transferase-positive cells was decreased.
12 - 十四酰佛波醇 - 13 - 乙酸酯（TPA）是一种强效肿瘤促进剂，可诱导人胸腺急性淋巴细胞白血病细胞系HPB - ALL发生表型分化。在有TPA存在的情况下接种后30分钟内，细胞形成光滑的圆形。在暴露于TPA 7天后，大多数细胞变得更小，类似大或非典型淋巴细胞。电子显微镜分析证明了TPA处理的HPB - ALL细胞的形态分化。用单克隆抗体OKT6染色的胸腺抗原显著减少，而在TPA处理的HPB - ALL细胞中Leu2a阳性细胞增加。然而，在这些TPA处理的细胞中，长达7天未出现OKT3阳性细胞。在TPA诱导的表型分化后，细胞的生长速率显著受到抑制，它们通过3H标记前体掺入DNA和RNA的能力降低，它们结合绵羊红细胞花环的能力显著增加，并且末端脱氧核苷酸转移酶阳性细胞的比例降低。
