Celiński A, Naskalski J W, Sznajd J
Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(2):201-12.
Ribonuclease (RNase) activity in the lymphocytes of 20 chronic lymphocytic leukemia (CLL) patients and 10 normal subjects was studied. It was found that in the lymphocytes of the control subjects the RNase activity could be detected in the pH range 4.5 to 8.6, inclusive. The RNase activity versus pH profile of normal lymphocytes consists of an acid RNase peak at pH 6.5 and alkaline RNase peak at pH 7.8. When treated with pCMB an inhibitor-bound RNase activity was revealed. The peak of this activity lay between pH 6.7 to 7.0. Liberating the inhibitor-bound RNase activity changed the RNase activity-pH profile, yielding one peak curve with a maximum at pH 7.0. RNase activity in CLL lymphocytes was remarkably lower than that in normal lymphocytes. The acid RNase in 80% of the CLL patients was lower by a factor of ten. Likewise, a many fold decrease in alkaline RNase activity (in some cases down to the zero level) was observed in CLL lymphocytes. However, in 70% of CLL patients, a level of the inhibitor-bound RNase activity was similar to that found in normal lymphocytes. In 20% of the studied CLL patients, a remarkable decrease in both free alkaline and inhibitor-bound RNase activity was observed. When poly-C was used as a substrate for determining RNase activity, a decrease to approximately 15% in CLL lymphocytes was observed, when poly-U was used instead of poly-C, a decrease to 65% was found only as compared with normal lymphocytes. This may suggest that CLL lymphocytes are deficient in a poly-C specific RNase which displays its activity within a neutral and alkaline pH range.
对20例慢性淋巴细胞白血病（CLL）患者和10名正常受试者淋巴细胞中的核糖核酸酶（RNase）活性进行了研究。结果发现，在对照受试者的淋巴细胞中，在pH值4.5至8.6（含）的范围内可检测到RNase活性。正常淋巴细胞的RNase活性与pH值的关系曲线包括在pH 6.5处的酸性RNase峰和在pH 7.8处的碱性RNase峰。用对氯汞苯甲酸（pCMB）处理后，显示出与抑制剂结合的RNase活性。该活性峰位于pH 6.7至7.0之间。释放与抑制剂结合的RNase活性改变了RNase活性与pH值的关系曲线，产生了一条在pH 7.0处有最大值的单峰曲线。CLL淋巴细胞中的RNase活性明显低于正常淋巴细胞。80%的CLL患者酸性RNase降低了10倍。同样，在CLL淋巴细胞中观察到碱性RNase活性有许多倍的下降（在某些情况下降至零水平）。然而，在70%的CLL患者中，与抑制剂结合的RNase活性水平与正常淋巴细胞中的相似。在20%的研究CLL患者中，观察到游离碱性RNase活性和与抑制剂结合的RNase活性均显著下降。当使用聚C作为测定RNase活性的底物时，CLL淋巴细胞中的活性下降至约15%，当使用聚U代替聚C时，仅与正常淋巴细胞相比下降至65%。这可能表明CLL淋巴细胞缺乏一种在中性和碱性pH范围内显示活性的聚C特异性RNase。
