Magloire H, Joffre A, Grimaud J A, Herbage D, Couble M L, Chavrier C
Histochemistry. 1982;74(3):319-28. doi: 10.1007/BF00493431.
The distribution of collagen type III throughout the pulp tissue from human developing tooth was studied using specific antibodies, immuno-fluorescence as well as immuno-peroxidase labelling for electron microscopy. Our results indicate that type III and type I collagen are present in the pulp. The staining intensity seems to correlate with the relatively high proportions of type III collagen biochemically found in pulp. In addition, type III collagen and reticulin fibres are similarly distributed, except that the Von Korff fibres were never detected with anti-type III collagen antibodies. Correspondingly, at the ultrastructural level, type III collagen appears as fine, branched filaments or electron dense material distributed throughout the tissue and particularly in close association with the plasma membrane of pulp fibroblasts. In contrast, type I collagen appears as typical coarse cross banded fibres.
利用特异性抗体、免疫荧光以及用于电子显微镜的免疫过氧化物酶标记技术，研究了Ⅲ型胶原蛋白在人类发育牙齿牙髓组织中的分布情况。我们的结果表明，Ⅲ型和Ⅰ型胶原蛋白存在于牙髓中。染色强度似乎与在牙髓中生化检测到的相对较高比例的Ⅲ型胶原蛋白相关。此外，Ⅲ型胶原蛋白和网状纤维的分布相似，只是用抗Ⅲ型胶原蛋白抗体从未检测到冯·科弗纤维。相应地，在超微结构水平上，Ⅲ型胶原蛋白表现为细小的分支细丝或电子致密物质，分布于整个组织中，尤其与牙髓成纤维细胞的质膜紧密相关。相比之下，Ⅰ型胶原蛋白表现为典型的粗横纹纤维。
