Dell'Orto P, Viale G, Colombi R, Braidotti P, Coggi G
J Histochem Cytochem. 1982 Jul;30(7):630-6. doi: 10.1177/30.7.6179982.
The postembedding immunoperoxidase staining technique for the localization of immunoglobulins (light and heavy chains) and of lysozyme has been successfully applied to epoxy-embedded human lymph nodes, after removal of the resin. Glutaraldehyde-containing fixatives appear to be suitable for the immunohistochemical localization of human immunoglobulins and lysozyme, provided that the masked antigenicity of these proteins is recovered by proteolytic digestion of the tissue sections using 0.4% pepsin or 0.1% trypsin. Nonglutaraldehyde-containing fixatives allow the immunolocalization of human immunoglobulins without any enzymatic pretreatment. This study shows that tissues routinely fixed in glutaraldehyde and embedded for ultrastructural investigations are actually suitable for immunohistochemical studies on human immunoglobulins and lysozyme.
在去除树脂后，用于免疫球蛋白（轻链和重链）及溶菌酶定位的包埋后免疫过氧化物酶染色技术已成功应用于环氧树脂包埋的人淋巴结。含戊二醛的固定剂似乎适用于人免疫球蛋白和溶菌酶的免疫组织化学定位，前提是通过用0.4%胃蛋白酶或0.1%胰蛋白酶对组织切片进行蛋白水解消化来恢复这些蛋白质被掩盖的抗原性。不含戊二醛的固定剂无需任何酶预处理即可实现人免疫球蛋白的免疫定位。本研究表明，常规用戊二醛固定并包埋用于超微结构研究的组织实际上适用于关于人免疫球蛋白和溶菌酶的免疫组织化学研究。
