支原体在小鼠脾细胞培养物中诱导干扰素的产生。

Induction of interferon by mycoplasmas in mouse spleen cell cultures.

作者信息

Beck J, Brunner H, Marcucci F, Kirchner H, Wietzerbin J

出版信息

J Interferon Res. 1982;2(1):31-6. doi: 10.1089/jir.1982.2.31.

DOI:10.1089/jir.1982.2.31

Abstract

Interferon production in cocultures between lymphoma cells and lymphocytes was previously found to be caused by mycoplasma contamination of the tumor cells. Here we have shown that M. arginini caused interferon production in spleen cells of C57BL/6 mice. The producer cell in spleen cell cultures was insensitive to treatment with anti-theta serum plus complement and it was present in spleen cell cultures of homozygous nude mice. Macrophage depletion did not adversely affect interferon production whereas interferon production was abolished in cultures of cells obtained after passage through nylon wool columns. Collectively, these data suggest, but cannot definitively prove that interferon is produced by B cells. By the use of specific antisera it was found that by all probability the interferon produced represented interferon alpha.

摘要

先前发现淋巴瘤细胞与淋巴细胞共培养时产生干扰素是由肿瘤细胞的支原体污染所致。在此我们已表明，精氨酸支原体可导致C57BL/6小鼠脾细胞产生干扰素。脾细胞培养物中的产生细胞对抗θ血清加补体处理不敏感，且存在于纯合裸鼠的脾细胞培养物中。巨噬细胞清除对干扰素产生没有不利影响，而通过尼龙毛柱传代后获得的细胞培养物中干扰素产生被消除。总体而言，这些数据提示但不能明确证明干扰素是由B细胞产生的。通过使用特异性抗血清发现，所产生的干扰素很可能代表α干扰素。