小鼠脾细胞培养物中单纯疱疹病毒诱导的干扰素产生细胞的研究。

Studies of the producer cell of herpes simplex virus-induced interferon in mouse spleen cell cultures.

作者信息

Kirchner H, Keyssner K, Zawatzky R, Hilfenhaus J

出版信息

Immunobiology. 1980 Dec;157(4-5):401-6. doi: 10.1016/S0171-2985(80)80009-X.

Abstract

The producer cell of type I interferon was studied in spleen cell cultures of C57BL/6 mice stimulated by inactivated Herpes Simplex Virus (HSV). Interferon production was not abolished by pretreatment of the spleen cells by anti-theta serum plus complement. The producer cell of interferon was not removed by plastic adherence and was not destroyed by the addition of silica. It was present in spleens of 3 day old C57BL/6 mice and in spleens of nu/nu mice. It was not inactivated by treatment of nu/nu spleen cells by anti-theta serum plus complement. HSV-induced interferon production was abolished by passage of the spleen cells through nylon wool columns and by irradiation (1000 R) of the spleen cells. Collectively these data suggest that in murine spleen cell cultures type I interferon is produced by B cells. However, our data do not allow to rule out that the interferon producing cell may be an immature macrophage or an immature T cell.

摘要

在经灭活单纯疱疹病毒（HSV）刺激的C57BL/6小鼠脾细胞培养物中，对I型干扰素的产生细胞进行了研究。用抗θ血清加补体预处理脾细胞，并不会消除干扰素的产生。干扰素产生细胞不会因塑料黏附而被去除，也不会因添加二氧化硅而被破坏。它存在于3日龄C57BL/6小鼠的脾脏以及无胸腺裸鼠的脾脏中。用抗θ血清加补体处理无胸腺裸鼠的脾细胞，并不会使其失活。脾细胞通过尼龙毛柱以及对脾细胞进行照射（1000伦琴）后，HSV诱导的干扰素产生被消除。总体而言，这些数据表明，在小鼠脾细胞培养物中，I型干扰素由B细胞产生。然而，我们的数据无法排除产生干扰素的细胞可能是未成熟巨噬细胞或未成熟T细胞的可能性。