小鼠淋巴瘤细胞对ATP的摄取

ATP uptake by mouse lymphoma cells.

作者信息

De Castro M F, Ayad S R

出版信息

Anticancer Res. 1982 Jan-Apr;2(1-2):41-5.

Abstract

Mouse lymphoma cells were shown to be unresponsive to prostaglandin E1 in terms of cAMP production. The endogenous ATP concentration was shown to be low. Addition of ATP in the presence of IMBX into the incubation medium resulted in elevation of the intracellular pool of ATP and the cells responded to PGE1 by increasing cAMP production. The ATP effect is specific and cannot be substituted by GTP. ATP analogue (AMP-PCP) can, however, produce a similar effect to ATP. The intracellular ATP concentration can be lowered by incubation with iodoacetate and potassium cyanide. This caused a drastic decrease of the cAMP level. Ethionine on the other hand had no effect on the intracellular ATP level.

摘要

小鼠淋巴瘤细胞在产生环磷酸腺苷（cAMP）方面对前列腺素E1无反应。内源性三磷酸腺苷（ATP）浓度较低。在存在异丁基甲基黄嘌呤（IMBX）的情况下向孵育培养基中添加ATP，导致细胞内ATP池升高，并且细胞通过增加cAMP产生对前列腺素E1作出反应。ATP的作用具有特异性，不能被鸟苷三磷酸（GTP）替代。然而，ATP类似物（AMP-PCP）可产生与ATP类似的作用。用碘乙酸盐和氰化钾孵育可降低细胞内ATP浓度。这导致cAMP水平急剧下降。另一方面，乙硫氨酸对细胞内ATP水平没有影响。