对听源性癫痫发作敏感的小鼠（弗林斯品系）大脑中神经化学参数的改变。

Altered neurochemical parameters in the brains of mice (Frings) susceptible to audiogenic seizures.

作者信息

Bakhit C, Shenoy A K, Swinyard E A, Gibb J W

出版信息

Brain Res. 1982 Jul 22;244(1):45-52. doi: 10.1016/0006-8993(82)90902-7.

Abstract

In this study, several biochemical aspects of serotonergic neurons were compared in mice susceptible to audiogenic seizures (Frings) and in mice not susceptible to audiogenic seizures (CF 1). Tryptophan hydroxylase (TPH) activity was significantly lower in brains of Frings mice compared to CF1 mice; however, brain levels of 5-HT were similar in both strains. The significantly lower TPH activity in Frings mice was due to the altered kinetic characteristics of TPH activity in this strain. TPH from Frings mice had a significantly lower apparent maximal velocity and a significantly higher affinity for the substrate, as indicated by a lower apparent Km for tryptophan. The uptake of tryptophan and accumulation of 5-HT (following pargyline) were similar in Frings and CF 1 mice. The levels of 5-HT in selected brain regions of Frings and CF 1 mice were also similar, but NE levels were higher in the cerebral cortex and DA levels were higher in the neostriatum of Frings mice. It is unclear at this point what role, if any, 5-HT plays in susceptibility to audiogenic seizures in brains of Frings mice.

摘要

在本研究中，对易患听源性癫痫的小鼠（弗林斯品系）和不易患听源性癫痫的小鼠（CF1品系）的血清素能神经元的几个生化方面进行了比较。与CF1小鼠相比，弗林斯小鼠大脑中的色氨酸羟化酶（TPH）活性显著降低；然而，两种品系小鼠大脑中的5-羟色胺（5-HT）水平相似。弗林斯小鼠中TPH活性显著降低是由于该品系中TPH活性的动力学特征发生了改变。弗林斯小鼠的TPH具有显著更低的表观最大速度以及对底物显著更高的亲和力，色氨酸的表观米氏常数（Km）更低即表明了这一点。弗林斯小鼠和CF1小鼠对色氨酸的摄取以及（服用帕吉林后）5-HT的积累情况相似。弗林斯小鼠和CF1小鼠选定脑区中的5-HT水平也相似，但弗林斯小鼠大脑皮层中的去甲肾上腺素（NE）水平较高，新纹状体中的多巴胺（DA）水平较高。目前尚不清楚5-HT在弗林斯小鼠大脑中对听源性癫痫的易感性中起到何种作用（如果有作用的话）。