小麦胚凝集素-辣根过氧化物酶复合物在加州海兔中的逆行轴突运输
Retrograde axonal transport of a wheat germ agglutinin-horseradish peroxidase conjugate in Aplysia californica.
作者信息
Kistler H B, Schwartz J H
出版信息
Brain Res. 1982 Jul 29;244(2):343-6. doi: 10.1016/0006-8993(82)90094-4.
To begin examining retrograde transport in a single invertebrate neuron we have used wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP), 18 h after the conjugate was injected extracellularly into the neuropil of the buccal ganglion of Aplysia, HRP reaction product could be detected cytochemically in putative lysosomes, cisternae and vesicles near Golgi in the giant metacerebral neuron (GCN) of the cerebral ganglion. Appearance of reaction product in the cell body was blocked by ligating GCN's axon and was inhibited by colchicine.
为了开始研究单个无脊椎动物神经元中的逆行运输，我们使用了与辣根过氧化物酶结合的小麦胚凝集素（WGA-HRP）。在将该结合物细胞外注射到海兔颊神经节的神经纤维网18小时后，可在脑神经元节的巨大脑神经元（GCN）中高尔基复合体附近的假定溶酶体、池和小泡中通过细胞化学方法检测到HRP反应产物。结扎GCN的轴突可阻断反应产物在细胞体中的出现，秋水仙碱也可抑制其出现。
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