De Rossi A, D'Andrea E, Chieco-Bianchi L
Tumori. 1982 Apr 30;68(2):95-104. doi: 10.1177/030089168206800201.
Endogenous XC- viruses were isolated from non-T spontaneous lymphomas of SJL/J(v+) mice. These isolates were cloned by limiting dilution procedure and characterized for biological and immunological properties. At the electron microscope these isolates showed typical retrovirus type C morphology. Reverse transcriptase and immunofluorescence assays showed that these viruses were infectious for mouse and heterologous cell lines. Since some of the isolates induced cytopathic foci in mink lung cultures, they had properties similar to those described for other recombinant dualtropic viruses. Using a panel of monoclonal antibodies against gp 70 and p 15 (E) viral proteins, it was observed that the dualtropic XC- viral isolates had an antigenic profile different from that of SJL ecotropic XC+ virus.
从SJL/J（v+）小鼠的非T自发性淋巴瘤中分离出内源性XC病毒。通过有限稀释法对这些分离株进行克隆，并对其生物学和免疫学特性进行表征。在电子显微镜下，这些分离株呈现出典型的C型逆转录病毒形态。逆转录酶和免疫荧光分析表明，这些病毒对小鼠和异源细胞系具有感染性。由于一些分离株在貂肺培养物中诱导产生细胞病变灶，它们具有与其他重组双嗜性病毒所描述的特性相似的性质。使用一组针对gp 70和p 15（E）病毒蛋白的单克隆抗体，观察到双嗜性XC病毒分离株的抗原谱与SJL嗜亲性XC+病毒不同。
