Brand S
Anat Embryol (Berl). 1981;162(4):475-88. doi: 10.1007/BF00301872.
The cellular composition of the primate claustrum was analyzed using serially sectioned Golgi impregnated neurons. The tissue used in this study was embedded in a soft resin mixture and cut with 25 mm long glass knives. The resin embedding allowed the sections to be cut serially at a thickness of only 3 micrometers. A camera lucida was employed for drawing the cellular processes from selected impregnated neurons; these drawings were later incorporated into a single composite picture of the neuron. Three types of neurons were observed in the primate claustrum. The largest of these neurons (Type 1) had a cell body and spine-laden dendritic arborization that varied in size and shape according to the neuron's position in the claustrum. The axons of Type I neurons were successfully impregnated in 25-day-old animals and were found to form collaterals within the claustrum. The collaterals from the axons of these cells appeared to leave the claustrum through both the external and extreme capsules. A second neuron found in the claustrum (Type II) had a round cell body with smooth beaded dendrites which radiated in all directions. The axon of the Type II neuron appeared to give off numerous collaterals that were not observed to leave the claustrum. A third type of neuron (Type III) had a small pear shaped cell body and a sparse dendritic tree. The axon and its collaterals appeared to remain within the dendritic circumference of the Type III neuron.
使用连续切片的高尔基浸染神经元对灵长类动物屏状核的细胞组成进行了分析。本研究中使用的组织嵌入软树脂混合物中，并用25毫米长的玻璃刀切割。树脂包埋使得切片能够以仅3微米的厚度连续切割。使用显微镜绘图仪绘制选定浸染神经元的细胞突起；这些绘图后来被整合到单个神经元的复合图片中。在灵长类动物屏状核中观察到三种类型的神经元。其中最大的神经元（1型）具有细胞体和充满棘突的树突分支，其大小和形状根据神经元在屏状核中的位置而变化。1型神经元的轴突在25日龄动物中成功浸染，并被发现在屏状核内形成侧支。这些细胞轴突的侧支似乎通过外囊和最外囊离开屏状核。在屏状核中发现的第二种神经元（2型）具有圆形细胞体，其光滑的串珠状树突向各个方向辐射。2型神经元的轴突似乎发出许多未观察到离开屏状核的侧支。第三种类型的神经元（3型）具有小梨形细胞体和稀疏的树突树。轴突及其侧支似乎保留在3型神经元的树突范围内。
