Hedrick S M, Matis L A, Hecht T T, Samelson L E, Longo D L, Heber-Katz E, Schwartz R H
Cell. 1982 Aug;30(1):141-52. doi: 10.1016/0092-8674(82)90020-4.
The activation of proliferative T lymphocytes normally involves the simultaneous recognition of a particular foreign antigen and a particular Ia molecule on the surface of antigen-presenting cells, the phenomenon of major histocompatibility complex (MHC) restriction. An analysis of T cell clones specific for pigeon cytochrome c, from B10.A and B10.S(9R) strains of mice, revealed the unusual finding that several of the clones could respond to antigen in association with Ia molecules from either strain. Using these cross-reactive clones, we performed experiments which demonstrated that both the Ia molecule and the T cell receptor contribute to the specificity of antigen recognition; however, MHC-linked low responsiveness to tuna cytochrome c (an immune response gene defect) could not be attributed solely to the efficacy with which the Ia molecules associated with the antigen. These results imply that antigen and Ia molecules are not recognized independently, but must interact at least during the process of T cell activation.
增殖性T淋巴细胞的激活通常涉及同时识别特定的外来抗原和抗原呈递细胞表面的特定Ia分子，即主要组织相容性复合体（MHC）限制现象。对来自B10.A和B10.S(9R)品系小鼠的针对鸽细胞色素c的T细胞克隆进行分析，发现了一个不寻常的结果：几个克隆能够与来自任一品系的Ia分子结合对抗原作出反应。利用这些交叉反应性克隆，我们进行了实验，结果表明Ia分子和T细胞受体都对抗原识别的特异性有贡献；然而，MHC连锁的对金枪鱼细胞色素c的低反应性（一种免疫反应基因缺陷）不能仅仅归因于与抗原结合的Ia分子的效力。这些结果表明，抗原和Ia分子不是独立被识别的，而是至少在T细胞激活过程中必须相互作用。
