赖氨酸衍生的交联存在于绿鬣蜥蛋壳的一种非弹性、富含脯氨酸的蛋白质组分中。

Lysine derived cross-links are present in a non-elastin, proline-rich protein fraction of Iguana iguana eggshell.

Cox D L, Mecham R P, Sexton O J

Comp Biochem Physiol B. 1982;72(4):619-23. doi: 10.1016/0305-0491(82)90515-6.

Abstract

Eggshell of Iguana iguana contains a variety of lysine-derived cross-linking amino acids apparently identical with some that occur in elastin. 2. Quantification of desmosine by amino acid analysis and radioimmunoassay gave estimates of 217 ng per mg and 109 ng per mg (dry weights), respectively. 3. [3H]NaBH4 reduction of eggshell protein established presence of lysine-derived aldehydes, allysine and allysine-aldol. 4. Enzymatic and chemical treatments indicate these cross-links occur in a non-elastin eggshell protein. 5. Iguana eggshell protein is unusually proline-rich, containing almost 440 proline residues per thousand.

摘要

绿鬣蜥的蛋壳含有多种赖氨酸衍生的交联氨基酸，显然与弹性蛋白中出现的某些氨基酸相同。2. 通过氨基酸分析和放射免疫测定对异锁链素的定量分别得出每毫克217纳克和每毫克109纳克（干重）的估计值。3. 用[3H]硼氢化钠还原蛋壳蛋白确定了赖氨酸衍生醛、烯赖氨酸和烯赖氨酸缩醛的存在。4. 酶促和化学处理表明这些交联存在于一种非弹性蛋白的蛋壳蛋白中。5. 绿鬣蜥蛋壳蛋白富含脯氨酸，异常独特，每千个氨基酸中几乎含有440个脯氨酸残基。