Pfeifle B, Ditschuneit H H, Ditschuneit H
Horm Metab Res. 1982 Aug;14(8):409-14. doi: 10.1055/s-2007-1019031.
Insulin-like growth factors (IGF) were isolated from human serum and compared with some biological actions of IGF supplied by Dr. J. Hapf, Zürich. Both factors were potent mitogens. They stimulated DNA-, RNA- and protein synthesis in cultivated human arterial smooth muscle cells. Furthermore, they enhanced the aminoacid transport. Our protein fraction (IGF Ulm) had a more potent biological activity than IGF (Zürich). Specific binding receptors for IGF (Zürich) on human arterial smooth muscle cells could be demonstrated. Specific binding of 125I-IGF (Zürich) was 10%. Half-maximal displacement was achieved by 250 ng/ml of unlabeled IGF (Zürich), by 1.2 micrograms/ml of IGF (Ulm), by 6.3 micrograms/ml of pro-insulin and by 17.8 micrograms/ml of insulin. In separate studies we could demonstrate that sera of normal adults, diabetic, acromegalic and hypophysectomized patients showed different growth-promoting activity in human arterial smooth muscle cells.
胰岛素样生长因子（IGF）从人血清中分离出来，并与苏黎世的J. Hapf博士提供的IGF的一些生物学作用进行了比较。两种因子都是有效的促有丝分裂原。它们刺激培养的人动脉平滑肌细胞中的DNA、RNA和蛋白质合成。此外，它们增强了氨基酸转运。我们的蛋白质组分（乌尔姆IGF）比IGF（苏黎世）具有更强的生物活性。可以证明人动脉平滑肌细胞上存在IGF（苏黎世）的特异性结合受体。125I-IGF（苏黎世）的特异性结合率为10%。250 ng/ml的未标记IGF（苏黎世）、1.2 μg/ml的IGF（乌尔姆）、6.3 μg/ml的胰岛素原和17.8 μg/ml的胰岛素可实现半数最大置换。在单独的研究中，我们可以证明正常成年人、糖尿病患者、肢端肥大症患者和垂体切除患者的血清在人动脉平滑肌细胞中表现出不同的促生长活性。
