Kugler P
Histochemistry. 1982;75(2):215-8. doi: 10.1007/BF00496013.
Fluorescence demonstration of cathepsin B (E.C. 3.4.22.1) activity was performed in the yolk sac of rats near term (18th day of gestation). The enzyme demonstration was performed on freeze-dried and celloidin mounted yolk-sac sections using different substituted beta-naphthylamide derivatives as substrates and nitrosalicylaldehyde as coupling agent. The discrete reaction products are localized preferentially in the apical part of the visceral yolk-sac epithelium. There is little doubt that cathepsin B is contained here in the well developed lysosomal apparatus of the yolk-sac epithelium.
在接近足月（妊娠第18天）的大鼠卵黄囊中进行了组织蛋白酶B（E.C. 3.4.22.1）活性的荧光检测。使用不同取代的β-萘酰胺衍生物作为底物，亚硝基水杨醛作为偶联剂，对冻干并火棉胶包埋的卵黄囊切片进行酶检测。离散的反应产物优先定位在内脏卵黄囊上皮的顶端部分。毫无疑问，组织蛋白酶B存在于卵黄囊上皮发育良好的溶酶体装置中。
