Bennett C E, Joshua G W, Hughes D L
J Parasitol. 1982 Oct;68(5):791-5.
A hyperimmune serum was raised in rabbits against soluble antigens of Fasciola hepatica metacercariae. This was subsequently absorbed with antigens from adult flukes and examined by immunoelectrophoresis (IEP) and by an indirect fluorescent antibody test (IFAT). When the absorbed antiserum was tested with F. hepatica metacercarial homogenate and adult homogenates by IEP, three distinct arcs appeared against the metacercarial homogenate and none against the adult homogenate. When tested by IFAT against a developmental series of flukes obtained from mice, the absorbed antiserum was positive on surfaces of whole flukes up to 4 days postinfection (PI) and on the tegument of sectioned flukes up to 7 days PI. Thus juvenile-specific antigens were demonstrated in the tegument of young (up to 7 days in mice) F. hepatica.
用肝片吸虫后尾蚴的可溶性抗原在兔体内制备了超免疫血清。随后用成虫的抗原对其进行吸收，并通过免疫电泳（IEP）和间接荧光抗体试验（IFAT）进行检测。当用IEP用吸收后的抗血清检测肝片吸虫后尾蚴匀浆和成虫匀浆时，针对后尾蚴匀浆出现了三条明显的弧，而针对成虫匀浆则没有。当用IFAT对从小鼠获得的一系列发育阶段的吸虫进行检测时，吸收后的抗血清在感染后4天内对整个吸虫的表面呈阳性，在感染后7天内对切片吸虫的体表呈阳性。因此，在幼龄（在小鼠体内长达7天）肝片吸虫的体表中证实了幼虫特异性抗原。
